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优秀论文选集（三）

拟交流类型：PosterPresentation

DecreasedexpressionofGDF9andBMP15genesinfolliclefluidandgranulosacellsderivedfromyoungpoorovarianrespondersundergoingIVF-ETtreatment

YanGong1,4,JesseLi-Ling3,Fang-yiLong1,4,Jia-jingWei1,4,JiajiangWei1,KunZhang2,Ya-nanZhang1,4,Jiu-zhiZeng1,41.SichuanProvincialWomen’sandChildren’sHospital2.ChengduMedicalCollege3.WestChinaHospital4.TheAffiliatedWomen’sandchildren’sHospitalofChengduMedicalCollege

ObjectiveTodeterminetheexpressionofgrowthdifferentiationfactor9(GDF9)andbonemorphogeneticprotein15(BMP15)genesinfolliclefluid(FF)andgranulosacells(GCs)derivedfromyoungwomenwithpoorovarianresponse(POR)undergoinginvitrofertilizationandembryotransfer(IVF-ET)treatment.

MethodsAprospectivecohortstudywascarriedoutbyenrolling65patientswithPORstratifiedaccordingtothePOSEIDONclassificationgroup3(age35,poorovarianreserveparameters)(PORgroup)and61age-matchedpatientswithoutPOR(controlgroup).AllpatientsunderwentIVF-ETtreatment.FFandGCswerecollectedafteroocyteretrieval.Enzymelinkedimmunosorbentassay(ELISA)wasusedtodeterminetheexpressionoftheGDF9andBMP15genesintheFF.Quantitativereal-timepolymerasechainreaction(qRT-PCR)andWesternblottingwereusedtodeterminetheexpressionoftheGDF9andBMP15geneinGCs.Thevaluesandout